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Primers for the amplification of rat genes

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described in detail in Table 1. The amplification programme consisted of an initial denaturation at 92C for 2minutes followed by 40 cycles from 92C for 15seconds, annealing at 55 62C, depending on the gene, for 30seconds and extension at 72C for 15seconds. PCR analysis was done in triplicate, with each set of assays repeated three times.

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