Primers for the amplification of rat genes

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described in detail in Table 1. The amplification programme consisted of an initial denaturation at 92C for 2minutes followed by 40 cycles from 92C for 15seconds, annealing at 55 62C, depending on the gene, for 30seconds and extension at 72C for 15seconds. PCR analysis was done in triplicate, with each set of assays repeated three times.

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Cho and colleagues demonstrated that working overtime had an effect on perceived patient safety and care left undone. The unpredictability of patient care may contribute to a longer shift, as nurses feel a professional obligation to complete their work and ensure safe care prior to finishing their shift. Therefore, hospital administrators may not necessarily be requiring the nurse to work longer shifts, but rather the inherent desire of nurses to ensure that their patients are safe, and all care has been delivered prior to leaving work, may be the driving factor behind this overtime.

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